MHC Monomers from Tetramer Store: Versatile Tools for Immunological Research
Tetramer Store’s Class I MHC Monomers offer researchers highly flexible tools for a wide range of immunological applications. Our monomers are biotin-labeled, making them ideal for use in specialized immunoassays, both in solid and solution-based formats. These reagents can be used to measure interactions between peptide-MHC (pMHC) complexes and monoclonal antibodies, recombinant T cell receptors (TCRs), or other pMHC-binding molecules. This makes them highly useful for screening antigen-specific CAR T cell receptors and other immunotherapies.
Stabilized Empty MHC Complexes: Flexibility and Efficiency
Our proprietary stabilized empty MHC monomers represent a significant advancement in flexibility and efficiency for rapid screening of multiple pMHC specificities. Researchers can easily load these monomers with a wide variety of peptides, enabling quick and efficient experiments in T cell immunology, antigen-specific CAR T development, and immune profiling.
Applications of Biotinylated MHC Monomers
MHC monomers from Tetramer Store are designed for numerous cutting-edge applications, including:
- Single Cell TCR Immune Profiling: Use MHC monomers in workflows to identify and characterize T cell receptor (TCR) specificities at the single-cell level, providing insights into immune responses.
- Customizable MHC Tetramers: Create specialized MHC tetramers with alternative labels, such as DNA-barcoded or metal-labeled MHC tetramers, to tailor detection to your specific needs.
- Antigen Fishing: Employ empty MHC monomers for antigen fishing assays, where the monomers are loaded with antigen and coupled with mass spectrometry (MS) to detect antigen-specific immune responses.
- Plate or Chip Coating for Assays: Biotinylated MHC monomers can be used to coat plates or chips, enabling high-throughput screening of pMHC specificity in assays.
- Microfluidics-Based T Cell Assays: MHC monomers are compatible with microfluidic systems for T cell assays, facilitating rapid, small-scale testing and analysis.
- Immunization Studies: These monomers can also be employed in immunization protocols to raise TCR-like antibodies, expanding their utility in vaccine development and antibody research.
Like Empty MHC tetramers, Empty MHC monomers are provided in a stabilizing formulation that preserves their peptide-receptive state. To maintain optimal performance, handling time should be minimized once the monomers are diluted out of this formulation, and the intended loading peptide should be added as soon as the monomers are introduced into downstream assays.
Why Choose Tetramer Store’s MHC Monomers?
- High Stability: Our MHC monomers are stabilized for optimal performance, ensuring reliable and reproducible results in every experiment.
- Customizable Flexibility: Load with your peptide of choice to screen multiple specificities, providing a versatile tool for research in immunotherapy, vaccine development, and more.
- Biotin Labeling for Versatile Use: The biotinylation of our MHC monomers allows for easy adaptation to various assays, such as solid-phase, solution-phase, or microfluidic systems.
Ordering MHC Monomers
Browse our webshop for a selection of biotinylated MHC monomers. We offer rapid worldwide shipping and custom options to meet your research requirements. Whether for basic research, drug development, or cutting-edge immunotherapy research, Tetramer Store’s MHC monomers offer the precision and flexibility you need.