Melanoma is a common form of skin cancer that arises in melanocytes, the cells responsible for producing melanin. While melanoma often begins in moles, it can also originate in other pigmented tissues such as the eyes or intestines, potentially spreading to other parts of the body.
Melanoma patients often develop an initial T cell response against tumor cells, but these T cells can become ineffective due to local immunosuppressive processes within the tumor. Recent advancements in immunotherapy, including FDA-approved drugs such as ipilimumab (Yervoy®), pembrolizumab (Keytruda®), nivolumab (Opdivo®), and T-VEC (Imlygic®), have been used to re-invigorate the immune system’s ability to attack melanoma.
These therapies rely on the reactivation of T cells targeting melanoma antigens. Tetramer assays have been instrumental in identifying T cells specific to a variety of melanoma-associated antigens, such as melanocyte lineage proteins like Tyrosinase, gp100, MART-1, and Cancer-Testis antigens like MAGE proteins and NY-ESO-1.
Melanoma-Specific Tetramers from Tetramer Store
Tetramer Store offers a comprehensive range of Tetramers designed to detect CD8+ melanoma antigen-specific T cells in peripheral blood and tumor-infiltrating lymphocytes (TILs). Our Tetramers target antigens such as MART-1, NY-ESO-1, MAGE-A3, Tyrosinase, gp100, and MAGE-A1, presented in MHC contexts including HLA-A02:01, HLA-A24:02, and HLA-B07:02.
Application and Benefits
- Key Applications: Detecting and isolating antigen-specific CD8+ T cells in research related to cancer vaccines and immunotherapies.
- Tetramer Customization: We offer Tetramers in a variety of fluorochromes including PE, BV421, APC, and many more. Custom colors are available upon request.
- Empty Loadable Tetramers: For maximum flexibility, Tetramer Store also supplies Empty Loadable Tetramers, enabling researchers to instantly load peptide antigens and create their own custom Tetramers.
Ordering Information
Visit our webshop for a full list of melanoma-specific Tetramers and control Tetramers. Our products are available for quick worldwide shipping with customizable options to meet your research needs.
Relevant melanoma-specific Tetramers – To order Tetramers >> Press here
Cat. No. | HLA type | Pep. Seq. | Antigen |
HA02-001 | HLA-A*02:01 | ELAGIGILTV | MelanA / MART 26-35 |
HA02-002 | HLA-A*02:01 | SLLMWITQV# | NY-ESO-1 157-165 |
HA02-003 | HLA-A*02:01 | SLLMWITQC# | NY-ESO-1 157-165 (9C) |
HA02-004 | HLA-A*02:01 | KVAELVHFL | MAGEA3 112-120 |
HA02-005 | HLA-A*02:01 | YMDGTMSQV | Tyrosinase 369-377 (371D) |
HA02-006 | HLA-A*02:01 | IMDQVPFSV | gp100 (pmel17) 209-217 |
HA02-019 | HLA-A*02:01 | ITDQVPFSV | gp100 (pmel) 209-217 |
HA02-007 | HLA-A*02:01 | KVLEYVIKV | MAGEA1 278-286 |
HA24-001 | HLA-A*24:02 | AFLPWHRLF | Tyrosinase 188-196 |
HA24-022 | HLA-A*24:02 | VYFFLPDHL | gp100-intron 4 (170-178) |
HB07-006 | HLA-B*07:02 | LPWHRLFLL | Tyrosinase 208-216 |
# It has previously been demonstrated that substitution of the cysteine to valine at position 165 (Chen, J.L., et al. 2000. Identification of NY-ESO-1 peptide analogues capable of improved stimulation of tumor-reactive CTL. J. Immunol. 165:948–955.) enhances the ability of the epitope to be recognized in vitro by NY–ESO-1157–165–specific CTL’s.
Therefore some researchers prefer to use the mutated peptide NY–ESO-1157–165 SLLMWITQV over the wild type peptide NY–ESO-1157–165 SLLMWITQC.
Control Tetramers
Cat. No. | HLA type | Pep. Seq. | Antigen |
HA02-024 | HLA-A*02:01 | AVIAPVHAV | None natural found |
HA02-025 | HLA-A*02:01 | GVAGDVSAV | None natural found |
HA02-014 | HLA-A*02:01 | SLYNTVATL | HIV-1 gag p17 76-84 |
Application of melanoma-specific Tetramers
Tetramers are useful in tetramer protocols for detection, enumeration and isolation of antigen-specific CD8+ T cells in a variety of applications e.g. during cancer vaccine – and immunotherapeutic development.
General information about MHC Tetramers from Tetramer Shop
Tetramer Store supply MHC Tetramers in various test sizes and the colors PE, BV421, BUV395, APC-Cy™7, APC, BV510, BV480, PE-Cy™5, PE-CF594, PE-Cy™7, PerCP-Cy™5.5, PerCP, FITC, BV605, BV650, BV786, APC-R700, BB515 and BUV737. For other colors, please enquire.
Tetramer Store also supply Empty Loadable Tetramers for instant loading of peptide antigens essentially representing DIY Tetramers.