PE and APC-Conjugated Streptavidin-Dextran MHC Multimers

Our PE Dextran and APC Dextran MHC Multimers offer a high-quality, affordable solution for researchers studying antigen-specific T cell populations. Designed as a competitive alternative to Immudex’s MHC Dextramers™, our multimers provide highly multivalent and flexible antigen presentation, optimized for sensitive detection in live cell assays and cutting-edge single-cell applications.

 

Why Choose Our Generic PE Dextran and APC Dextran MHC Multimers?

Built on a 500 kDa amino dextran backbone, our multimers ensure excellent hydrophilicity and flexibility, allowing effective presentation of antigenic peptides for flow cytometry. Each dextran molecule carries 4 streptavidin molecules and 3 PE or 6 APC fluorophores, ensuring bright, reliable detection of antigen-specific T cells.

 

Additionally, the dextran scaffold carries up to 10 MHC molecules, allowing ample space for attaching additional biotinylated molecules like biotinylated DNA barcodes such as TotalSeq™ C DNA barcodes from BioLegend or similar barcodes. This feature enables the use of our multimers in single-cell T cell discovery, adding unparalleled value for researchers in the rapidly growing field of high-dimensional immune profiling.

 

Key Features:

  • Fluorescent Streptavidin-Dextran Conjugates: With a 500 kDa amino dextran backbone, our multimers provide a flexible, hydrophilic scaffold that supports efficient MHC-peptide complex presentation and multivalency.
  • Enhanced Single-Cell Detection: The ability to attach biotinylated DNA barcodes, like TotalSeq™ C DNA barcodes from BioLegend or similar barcodes, onto the conjugates opens up avenues for single-cell sequencing studies, enabling the deep profiling of T cell receptor specificities at the single-cell level.
  • High Sensitivity and Multivalency: The multiple streptavidin sites on each dextran backbone enhance the detection of low-affinity T cell receptor interactions with MHC-peptide complexes.
  • Live Cell Compatibility: Extensively tested for live cell staining, our multimers provide reliable performance in standard flow cytometry assays as well as more advanced single-cell studies.

 

Comparison to Immudex’s MHC Dextramers™:

Immudex’s MHC Dextramers™ set a high bar for multimer-based T cell detection, but our generic multimers offer similar functionality, plus added features, at a more accessible price point:

  • Multivalency and Fluorescence: Both products offer highly multivalent MHC-peptide presentations and are conjugated to PE or APC for clear, bright signals in flow cytometry.
  • Single-Cell Versatility: Our multimers’ unique capability to carry biotinylated DNA barcodes gives them an advantage in single-cell applications, providing greater flexibility for researchers looking to integrate flow cytometry with next-generation sequencing technologies.

 

Technical Specifications:

  • Dextran Backbone: 500 kDa amino dextran
  • Number of Streptavidin Molecules: 4 per dextran molecule
  • Number of Fluorophores (PE or APC): 3 or 6, respectively, per dextran molecule
  • Average MHC Molecules per Conjugate: 10
  • Applications: Flow cytometry for live cell staining, T cell detection, and advanced single-cell discovery with biotinylated DNA barcodes.

 

Conclusion

Our PE and APC-conjugated Streptavidin-Dextran MHC Multimers offer a high-performance, cost-effective alternative to Immudex’s MHC Dextramers™. With their enhanced ability to carry biotinylated DNA barcodes, they provide a powerful tool for single-cell T cell discovery. Whether you’re performing standard flow cytometry or exploring advanced single-cell sequencing, our multimers deliver the sensitivity and flexibility you need—at a fraction of the cost.

 

For more information, pricing, or to place an order, contact us below!